ISHS Acta Horticulturae 637: XXVI International Horticultural Congress: Advances in Vegetable Breeding
CREATION OF A NEW GERMPLASM OF CMS NON-HEADING CHINESE CABBAGE |
| Authors: | X.-L. Hou, S.-C. Cao, Y.-K. He |
| Keywords: | non-heading Chinese cabbage, somatic hybrid, cytoplasm male sterile, mitochondrial DNA (mtDNA), chloroplast DNA (cpDNA) |
| Abstract:
A new germplasm ZS6(A)10 was created by asymmetric cell fusion with progeny plants identified in both field and laboratory. Results showed that the material displayed 100% sterility rate, 100% sterility degree, it had 4 nectaries and no chlorosis at low temperature. The seed-set was identical to that of the maintainer, which was significantly higher than that of the parental sterile line. The chromosome number of the progeny plant was the same as the maintainer (2n=20). The POD isoenzyme of the radicle and cotyledons, and the EST isoenzyme of the hypocotyl were notably different with those of the maintainer and the parental sterile line. The cpDNA and mtDNA contents of the progeny plants were intermediate between the maintainer and the parental sterile line. PCR analysis of cpDNA and mtDNA confirmed that ZS6(A)10 was a somatic hybrid and its progeny 98HQiu-45 was a new germplasm of Cytoplasmic Male Sterile (CMS) non-heading Chinese Cabbage. | |
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