ISHS Acta Horticulturae 637: XXVI International Horticultural Congress: Advances in Vegetable Breeding
GENETIC ANALYSIS OF PROCESSING TRAITS IN GREEN BEAN (PHASEOLUS VULGARIS) |
| Authors: | J.R. Myers, J.W. Davis, B. Yorgey, D. Kean |
| Keywords: | snap bean, Random Amplified Polymorphic DNA, RAPD, Molecular markers |
| Abstract:
A recombinant inbred population consisting of 80 lines was developed from the cross ‘Minuette’ X ‘OSU 5630’. OSU 5630 is an Oregon bush blue lake standard sieve green breeding line and Minuette is a small sieve cultivar developed by Harris-Moran. The two cultivars exhibited contrasting traits for pod size and length, cross-section, color, shininess, as well as for plant architecture and a cripple phenotype resulting from a Mesoamerican - Andean center of origin interaction. A test of phaseolin seed storage protein type revealed that OSU 5630 possessed S-phaseolin and Minuette had T-phaseolin. This result was unexpected because most North American green beans have T-phaseolin. The lines with parents were grown in a randomized complete block replicated twice in the field in 2001. Plots were hand-picked at processing maturity, and evaluated for sieve size distribution, pod color, length, cross-section, smoothness, straightness, strings, and shininess. Pods were then blanched and frozen for additional characterization of processing traits. Pod shininess (conditioned by ace) segregated as expected for a single qualitative gene, while other traits had quantitative inheritance. While both parents have round pod cross-section and are stringless, the progeny segregated for flat and oval pod cross-section as well as strings, suggesting that different genes control those traits in these two lines. The plant architecture traits (plant height, internode length, branching, pod distribution, and main stem thickness) all exhibited quantitative inheritance. Efforts are underway to develop a molecular marker map using this population, and map QTL associated with processing and plant architecture characters. | |
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