ISHS Acta Horticulturae 637: XXVI International Horticultural Congress: Advances in Vegetable Breeding
ANALYSIS OF GENETIC RELATIONSHIPS OF WATERMELON [CITRULLUS LANATUS (THUNB.) MANSFELD] GERMPLASM USING RAPD AND AFLP |
| Authors: | Y. Xu, J. Guo, H. Zhang, G. Gong, K.-P. Che, B. Wang |
| Keywords: | watermelon, genetic relationship |
| Abstract:
Random Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) analysis was applied in assessing genetic relationships among 30 accessions of watermelon [Citrullus lanatus (Thunb.) Mansf.] that represent a wide range of breeding and commercially available germplasm, including cultivated and wild accessions. Fifteen primers (2.08%) were screened from 720 arbitrary 10-mer primers, and a total of 104 DNA bands were amplified, 43 of which (41.35%) were polymorphic. The average number of DNA bands amplified by each primer was 7.0. Among bands generated by eight selected AFLP primer combinations (among 24 primer combinations), 13.0%-31.9% were polymorphic among the cultivars, while 45.3%-64.2% were polymorphic among all the accessions. DNA fingerprinting based on 15 bands amplified with four primer combinations E-AT/M-CTA, E-AG/M-CAT, E-AT/M-CAT, E-AA/M-CAA was performed and utilized in watermelon genotype identification. A tree diagram, constructed using the complete linkage method, resulted in six groups: one East-Asian group, one American group, two medial groups, and two African wild groups. Each ecological group exhibited specific bands, which were useful in discriminating other groups. Results of cluster analysis based on the RAPD and AFLP data were consistent with and verified previous classification of the germplasm. | |
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