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Authors: | D.D. Archbold, M. Nosarzewski, A.M. Clements, A.B. Downie |
Keywords: | carbohydrate metabolism, enzyme, Malus x domestica |
DOI: | 10.17660/ActaHortic.2004.636.53 |
Abstract:
Accumulation of sorbitol by developing apple fruit is critical to achieving size and quality.
The primary enzyme involved in sorbitol accumulation is NAD-dependent sorbitol dehydrogenase (SDH; EC 1.1.1.14), converting sorbitol to fructose.
SDH activity varies seasonally but can be manipulated by altering sorbitol availability.
Possible transcriptional and translational regulation of SDH activity are being studied.
Using activity assays and an SDH-specific antibody, an analysis of seasonal variation has revealed SDH activity and protein immediately following bloom, during the critical fruit set period.
This is in addition to previously reported SDH activity peaks at 6 to 8 weeks after bloom and at ripening.
Does the presence of SDH at this early time, not previously reported, have significant implications with respect to fruit set in apple?
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