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| Authors: | S.P.O. Werbrouck, P.C. Debergh |
| Keywords: | Arabidopsis, brassinolide, de-etiolation, gibberellic acid |
Abstract:
Dark germinating Arabidopsis thaliana seedlings showed a clearly de-etiolated phenotype on a medium with 2.5 μM imazalil (IMA). Comparable with brassinosteroid deficient mutants, hypocotyl length was reduced and cotyledons had opened.
A major part of the hypocotyl length reduction could be restored by 0.1 μM epibrasinolide (EBR). Combining EBR with 2.5 μM GA3 was necessary for a complete restoration of the hypocotyl length.
The dose dependent root length reduction by IMA could not be overcome by adding EBR. These experiments suggest that one of the side effects of IMA is the inhibition of the brassinosteroid biosynthesis.
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