ISHS Acta Horticulturae 635: XXVI International Horticultural Congress: Managing Soil-Borne Pathogens: A Sound Rhizosphere to Improve Productivity in Intensive Horticultural Systems
BIOLOGICAL CONTROL OF ROOT KNOT NEMATODES (MELOIDOGYNE SPP.) ON TISSUE CULTURE BANANA (DWARF CAVENDISH VAR. BASARAI) |
| Authors: | O. Fadina Olubunmi, S. Nadgauda Rajani |
| Keywords: | Active ingredients, toxic metabolites, Solanum melongena, juveniles, mortality |
| Abstract:
Biocontrol powder Phule Trichoderma has been successfully used on a number of horticultural crops. The present investigation was carried out to explore the nematicidal properties of Phule Trichoderma against the root-knot nematode (Meloidogyne spp.) infesting the tissue culture banana (Dwarf Cavendish - var. Basarai). In vitro tests showed that the various concentrations of Phule Trichoderma prevented nematode egg hatching and also resulted in 100% mortality of nematode juveniles. Tissue culture banana plants were also dipped into various concentrations of Phule Trichoderma before planting out into plastic bags. Plant were inoculated with 250 nematode juveniles and after ten days, the roots were stained with cotton blue lactophenol and nematodes were counted under a dissecting microscope. The results indicated that the higher the concentration of Phule Trichoderma in banana plants, the lower the ability of the nematode to penetrate the roots. Furthermore, the nematode juveniles that penetrated the treated roots were found dead. This could have resulted from the toxic metabolites produced from Phule Trichoderma. Also, root zone treatment of plants treated with Phule Trichoderma prevented the development of giant cells and roots knots in treated plants while the development of giant cells and root knots were observed in untreated plants exposed to nematode infestation. | |
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