ISHS Acta Horticulturae 634: XXVI International Horticultural Congress: IV International Symposium on Taxonomy of Cultivated Plants
RAPD-PCR ANSWERS SOME LONG-STANDING QUESTIONS ABOUT ROSE IDENTIFICATION |
| Authors: | M.M. Manners, N. Morvillo, C. Frederick, A. Wagner |
| Keywords: | Rosa moschata, Noisette, DNA analysis |
| Abstract:
In the past 20 years, there has been a resurgence of interest in “historic,” “heritage,” or “antique” rose cultivars. Numerous commercial nurseries now specialize in producing old roses. In many cases, there is little or no evidence that the rose currently being grown is the cultivar historically connected with the name being used. In some cases, a rose sold under a historic name by one nursery may not be the same cultivar as is being sold under the same name by a different nursery. Also, historic records of roses’ parentage are not always accurate. This paper illustrates how DNA analysis using randomly amplified polymorphic DNA (RAPD) analysis via the polymerase chain reaction (PCR) can assist in answering such questions about the identity and relationships of old roses. The early members of the Noisette class were studied - the parentage of ‘Champneys’ Pink Cluster’, the relationship of that rose to ‘Blush Noisette’, and the relationship of the various forms of Rosa moschata currently in commerce. Also, accessions of ‘Champneys’ Pink Cluster’ and ‘Blush Noisette’ from multiple nurseries were compared, to determine if they are all the same clones. | |
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