ISHS Acta Horticulturae 633: XXVI International Horticultural Congress: Protected Cultivation 2002: In Search of Structures, Systems and Plant Materials for Sustainable Greenhouse Production
ENVIRONMENT CONTROL MODELS FOR A GROWTH STAGE-DEPENDENT PHOTOAUTOTROPHIC POTATO MICROPROPAGATION SYSTEM |
| Authors: | J.E. Son, H. Lee, Y. Jang |
| Keywords: | The growth of tissue-cultured plantlets under photoautotrophic |
| Abstract:
The growth of tissue-cultured plantlets under photoautotrophic conditions was sensitive to environment factors, and therefore adequate environment controls were required to ensure the growth and quality of the plantlets. In this study, the whole lifecycle of potato (Solanum tuberosum L. cv. Dejima) was divided into three stages and their environmental requirements were investigated. Single-node cuttings from potato, subcultured photomixotrophically in vitro, were grown in Magenta vessel. Dry and fresh weights, shoot length and diameter, and the number of nodes of potato plantlets were analyzed under 400, 700, and 1400 μmol mol-1 CO2 conditions with 80, 160, and 240 μmol m-2 s-1 PPF levels at the 1st stage, and under 700, 1400, and 1800 μmol mol-1 CO2 conditions with 80, 160, 240, and 320 μmol m-2 s-1 PPF levels at the 2nd-3rd stages. At the 1st stage, few differences in various growth factors were observed among all treatments, but at the 2nd-3rd stages, the differences were much greater. At the 3rd stage, for instance, fresh and dry weights showed relatively direct linearity with respect to PPF (80-320 μmol m-2 s-1) at 1400 μmol mol-1 CO2, but were non-linear with a saturated point around 240 μmol m-2 s-1 PPF at 1800 mol μmol-1 CO2. Using all the relationships among growth and environment factors, growth stage-dependent environment control models were investigated. | |
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