ISHS Acta Horticulturae 630: XXVI International Horticultural Congress: Nursery Crops; Development, Evaluation, Production and Use
RESISTANCE OF ROSES TO CROWN GALL DISEASE ARE RELATED TO THE QUANTITY OF ACETOSYRINGONE DERIVATIVES EXUDATES |
| Authors: | B.S. Tan, K. Hasuo, S. Kawai, K. Kageyama, S. Matsumoto, H. Fukui |
| Keywords: | Agrobacterium tumefaciens, resistance mechanism, 'PEKcougel', 'Lifirane', 'Dukat', Rosa multiflora, Rosa canina |
| Abstract:
Acetosyringone derivatives secreted by wounded plant cells induce activities of vir genes in Agrobacterium tumefaciens, ultimately causing tumorigenesis in the plant. This is what widely known as crown gall disease. It was reported that resistance to crown gall disease was various among roses, and was inherited as multiple genes, but the resistance mechanism remains unclear. To investigate the relation between acetosyringone derivatives secretion and resistance, the quantitative analysis of acetosyringone derivatives from leaf disks exudation of 2 resistant roses, ‘PEKcougel’ and ‘Lifirane’, and 3 susceptible roses, ‘Dukat’, Rosa multiflora and R. canina was performed. Acetosyringone was also detected qualitatively in R. multiflora by GC-MS and LC-MS. For quantitative analysis, a system for separation of 13 acetosyringone derivatives by HPLC was developed. Seven individual acetosyringone derivatives and three pairs were separated well under methanol 10% (0min), 16% (40min), 30% (60min) gradients conditions, on the ODS column. It was found that the resistant roses secreted less acetosyringone derivatives than the susceptible ones, in term of quantity. Thus it is concluded that at least part of the resistance mechanism of crown gall disease is related to acetosyringone derivatives exudation. | |
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