ISHS Acta Horticulturae 629: XXVI International Horticultural Congress: The Future for Medicinal and Aromatic Plants
METHYL JASMONATE INCREASES SAPONIN CONTENT IN BIOREACTOR CULTURE OF GINSENG (PANAX GINSENG C. A. MEYER) ADVENTITIOUS ROOTS |
| Authors: | Y. Kim, D. Chakrabarty, E. Hahn, K. Paek |
| Keywords: | Jasmonic acid derivatives, medicinal plants, secondary metabolites |
| Abstract:
Asian ginseng (Panax ginseng C. A. Meyer), considered one of the most potent traditional medicinal plants, has been used for centuries as a health tonic. The important bio-active components in ginseng root are the saponins, of which more than 20 have been identified. Current advances in plant biotechnology allowed production of saponins through adventitious root culture of ginseng in bioreactors. The adventitious root is an efficient means of saponin production due to fast root growth and stable metabolite productivity, although saponin production in culture is lower than that from field-cultivated ginseng root. To increase saponin content in culture, ginseng adventitious roots were cultured in a 20 l balloon type air-lift bioreactor for 40 days and treated with different types and concentrations of elicitors for 10 days. Treatment with 50 mmol methyl jasmonate for 7 days maximized saponin content. Saponin content started to increase from day 1 of treatment and peaked after 7 days of culture. The impact of salt concentrations in the culture medium and the effect of methyl jasmonate on saponin production requires further study. | |
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