ISHS Acta Horticulturae 629: XXVI International Horticultural Congress: The Future for Medicinal and Aromatic Plants
DNA FINGERPRINTING IN HYDRASTIS CANADENSIS USING RAPD ANALYSIS |
| Authors: | K.J. Kelley, J. Jung, A. Frary |
| Keywords: | Goldenseal, medicinal plant, wildflower |
| Abstract:
Hydrastis canadensis L. (goldenseal) is an endangered perennial wildflower species native to eastern North America. In this study, we analyze several populations of Hydrastis canadensis for genetic diversity within and among populations. The samples are from cultivated populations in Massachusetts and Canada and wild populations from Ohio, West Virginia and New York. The RAPD analysis technique was used to generate DNA profiles from individual plants and to estimate genetic relatedness within and between populations. Ready to Go RAPD Analysis Bead Kits were used for the amplifications to help ensure consistency and reproducibility. The results for the cultivated material show 72-86% similarity among the four populations tested and the wild populations 20-67% similarity. These numbers are in line with what is expected when comparing a cultivated group with the wild populations. An interesting result was the similarity between one Ohio population and the New York populations. These results imply that the New York population may have originated from cultivated material. Because there are disadvantages to having limited genetic variability, greater knowledge about the levels variation within the populations could assist in crop production and reintroduction strategies for this species. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
