ISHS Acta Horticulturae 629: XXVI International Horticultural Congress: The Future for Medicinal and Aromatic Plants
IMPLEMENTATION OF MOLECULAR TECHNIQUES (RAPDS, AFLPS) ON CAMOMILE (CHAMOMILLA RECUTITA (L.) RAUSCH.) FOR GENOTYPING AND MARKER DEVELOPMENT |
| Authors: | C. Wagner, R.A. Marquard, W. Friedt, F. Ordon |
| Keywords: | Bisabolol, genetic diversity, molecular markers |
| Abstract:
Camomile varieties of different provenience can be distinguished by morphological and physiological traits. However, these traits vary under different environmental conditions leading to difficulties in the discrimination of genotypes. PCR-based molecular techniques like RAPDs and AFLPs are useful tools to characterise genotypes rapidly and reliably on the DNA-level independently from ecological factors. Besides this, these techniques facilitate the development of molecular markers for ingredients of camomile oil, e.g. (-)-  -bisabolol, thereby enabling pre-flowering selection.Therefore, attempts were carried out to establish these molecular techniques on camomile. In a first step genetic similarity (Jaccard, 1908) was estimated in a set of released cultivars, breeding populations and twice self-pollinated lines. Based on data obtained by 20 RAPD primers and 16 AFLP EcoRI+3/MseI+3 primer combinations genetic similarity was estimated between 0.52 and 0.91 (RAPDs) and 0.58 to 0.79 (AFLPs). By cluster analysis as well as by principle co-ordinate analysis genotypes were grouped according to their different proveniences. Regarding the self-pollinated lines which differ in their (-)- -bisabolol content a high degree of homogeneity within lines (0.81-0.94) and a relatively low genetic similarity between them (0.46-0.56) was determined by RAPDs.
By crosses between these lines a segregating F2-population was created for developing molecular markers corresponding to the (-)--bisabolol content.
Using bulked segregant analysis (Michelmore et al., 1991) a set of 200 RAPD-primers and 256 AFLP-primer combinations were tested up to now and two AFLP-markers linked to the (-)--bisabolol content were detected.
Future work will aim at the development of additional more closely linked markers.
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