ISHS Acta Horticulturae 629: XXVI International Horticultural Congress: The Future for Medicinal and Aromatic Plants
A NOVEL APPROACH OF BACTERIA-FREE RHIZOME PRODUCTION OF GINGER THROUGH BIOTECHNOLOGY |
| Authors: | C. Kirdmanee, K. Mosaleeyanon, M. Tanticharoen |
| Keywords: | meristem culture, multiplication, rhizome production, Zingiber officinale Rosc. |
| Abstract:
Ginger buds were sterilized and cultured on Murashige and Skoog (MS, 1962) medium at 29±1 °C air temperature in the dark condition for 1 month. The meristems of developed shoots were cut into 0.15±0.05, 0.35±0.05, 0.55±0.05, and 0.75±0.05 mm in diameter and cultured on MS medium supplemented with 15 % coconut water for rapid screening of bacterial infection. The bacteria-free shoots were multiplied on MS medium supplemented with 0, 2, 4, and 6 mg l-1 N6-Banzylamine (BA) or 0, 2, 4, and 6 mg L-1 6-(  -dimethylallylamino) purine (2iP) at 25±1 °C air temperature for 5 weeks.
The ginger plantlets were transferred to ex-vitro and produced the bacteria-free rhizome.
The bacteria-free rhizomes were compared with those of rhizome produced through conventional method in the commercial scale.
The lowest bacterial infection of meristem was observed in the shoots cut into 0.15±0.05 mm in diameter.
The shoots multiplied on the medium supplemented with 4 mg L-1 BA were 1.9 times higher when compared with those multiplied on the medium supplemented without.
The vigorous growth, high survival percentage and high yield were observed in the ginger plants produced through bacteria-free rhizome.
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