ISHS Acta Horticulturae 629: XXVI International Horticultural Congress: The Future for Medicinal and Aromatic Plants
INSECTICIDAL EFFECTIVENESS OF COMPOUNDS FROM MAMMEA SIAMENSIS KOST. AGAINST MUSCA DOMESTICA LINN. |
| Authors: | K. Issakul, W. Kongtrakoon, S. Dheeranupatana, S. Jangsutthivorawat, A. Jatisatienr |
| Keywords: | Bioassay, biopesticide, bioinsecticide, natural product, natural compound, botanical pesticide |
| Abstract:
Insecticidal substances from dried seeds of Mammea siamensis Kost. extracted with dichloromethane and fractionated by TLC were investigated. The bioassay was done in laboratory to test for the insecticidal efficiency of those fractions to the egg, maggot, pupae, and adult of the house fly, Musca domestica (Linn.). The crude extract of M. siamensis was separated by TLC on a silica plate with toluene: ethylacetate (80:20) as mobile phase into 8 fractions.The fractions with Rf  0.61 and Rf 0.96 tended to express insecticidal affects to the egg of the insect.A total of 61.11% of the eggs did not develop into maggots.However, there was no significant difference among these studied fractions.The Rf= 0.96 fractions showed the best average number of 30 % of pupae which did not develop to adults.The Rf 0.96 and Rf 0.14 showed 51.67 % and 45.00 % mortality in to the adult after 4 h..
The Rf 0.14 and Rf= 0.96 were re-purified by TLC using mobile phase of toluene: ethylacetate (93:7) and tested for insecticidal efficiency on the adult.
The Rf= 0.32 ( from Rf= 0.14) and the Rf= 0.52 ( from Rf= 0.96) expressed the average number of 90 % and 95 % mortality, respectively, in 4 h
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