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| Authors: | T. Suzuki, Y. Sasaki, K. Kuroda, K. Oosawa |
| Keywords: | Cortex, Cryo-SEM, freeze-edging, ice crystal, intercellular space, pith |
Abstract:
In order to clarify the mechanism of water core development in apple fruit, changes in the development of watercore in fruit of three cultivars, either on trees or stored at 2 ˚C after harvest, were examined.
The water core developed in fruit of 'Koutoku' and 'Redgold', but not in 'Mutsu'. Formation of the water core was first confirmed in mid-October.
The size of the water core was maximum at harvest time, then reduced gradually in stored fruit.
Tissues of the fresh fruit were observed with a cryo-scanning electron microscope (Cryo-SEM), with which samples can be observed without dehydration.
The Cryo-SEM image showed that all the intercellular spaces were filled with ice crystals (aqueous fluid) in the water core tissue, but not in the non-water core area.
Water core tissue seems to be formed with aqueous fluid overflow in the apoplast just before harvest.
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