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ISHS Acta Horticulturae 628: XXVI International Horticultural Congress: Issues and Advances in Postharvest Horticulture

HISTOLOGICAL FEATURES AND DYNAMICS OF THE DEVELOPMENT OF WATERCORE IN APPLE (MALUS DOMESTICA) FRUIT

Authors:   T. Suzuki, Y. Sasaki, K. Kuroda, K. Oosawa
Keywords:   Cortex, Cryo-SEM, freeze-edging, ice crystal, intercellular space, pith
Abstract:
In order to clarify the mechanism of water core development in apple fruit, changes in the development of watercore in fruit of three cultivars, either on trees or stored at 2 ˚C after harvest, were examined. The water core developed in fruit of 'Koutoku' and 'Redgold', but not in 'Mutsu'. Formation of the water core was first confirmed in mid-October. The size of the water core was maximum at harvest time, then reduced gradually in stored fruit. Tissues of the fresh fruit were observed with a cryo-scanning electron microscope (Cryo-SEM), with which samples can be observed without dehydration. The Cryo-SEM image showed that all the intercellular spaces were filled with ice crystals (aqueous fluid) in the water core tissue, but not in the non-water core area. Water core tissue seems to be formed with aqueous fluid overflow in the apoplast just before harvest.

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