ISHS Acta Horticulturae 625: XXVI International Horticultural Congress: Biotechnology in Horticultural Crop Improvement: Achievements, Opportunities and Limitations
PLANT REGENERATION FROM LEAF EXPLANTS AND EFFICIENT AGROBACTERIUM-MEDIATED TRANSFORMATION SYSTEM OF CHRYSANTHEMUM (DENDRANTHEMA GRANDIFLORUM) |
| Authors: | S.Y. Seo, D.C. Choi, J.M. Kim, H.C. Lim, H.J. Kim, J.S. Choi, Y.G. Choi |
| Keywords: | growth regulators, nptII, kanamycin, PCR amplification, transgenic plant |
| Abstract:
Leaf explants of chrysanthemum (Dendranthema grandiflorum) were cultured on MS medium supplemented with various concentrations of NAA, IAA, 2,4-D and BA. The highest frequency of shoot formation was observed on MS medium supplemented with 1.0 mg L-1 BA, 0.3 mg L-1 2,4-D in Puma and with 2.0 mg L-1 NAA, 0.5 mg L-1 BA in Subangryeok. Suitable concentrations of kanamycin to select transformants were 20 mg L-1 in Puma, and 50 mg L-1 in Subangryeok. In Puma, the calli formation was most efficient in the medium containing kanamycin from leaf discs co-cultivated with A. tumefaciens inoculation at 1:50 dilution ratio for 5 min. PCR detection of the npt II gene gave a 700 bp band in two transformants. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
