MICROPROPAGATION OF ORNITHOGALUM SAUNDERSIAE BAK.

In micropropagation of Ornithogalum saundersiae Bak using scale pieces, combinations of 0, 0.05, 0.5 or 5μM NAA and 0, 0.5, 5, 10, or 20 μM BA in the culture medium were compared. Explants on media without NAA and BA did not survive while the highest survival of up to 93.3% was observed in medium with 5μM of both NAA and BA and up to 88.9% in medium with 0.5μM NAA and 10 μM BA. In two sets of experiments, 5 and 0.5 μM NAA were the best concentrations, yielding survival rates from 47.3 to 72.2%. Both NAA and BA had a significant effect on organ regeneration, and the interaction between them was highly significant. The percent of explants with shoots and the number of shoots generally increased with increasing NAA concentration but was not significantly affected by the concentration of BA. Roots were mainly formed on explants in media with concentrations of 0.5 or 5μM NAA while inclusion of BA inhibited root regeneration. Callus was formed on explants on media with 5μM of NAA, no matter the concentration of BA. Tissue culture of O. saundersiae from various floral explants was possible but they required 0.5 μM NAA and 2 μM BA in the MS medium to regenerate organs. Plantlets, shoots, roots and/or callus were obtained directly or indirectly (through intervening callus) from immature florets, scape and nodal sections, pedicels and sepals.

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