ISHS Acta Horticulturae 624: XXVI International Horticultural Congress: Elegant Science in Floriculture
PLOIDY IDENTIFICATION IN M1 AND M2 GENERATION OF VIOLA x WITTROCKIANA GAMS. AFTER MUTAGENESIS |
| Authors: | I. Ajalin, F. Kobza, J. Doležel |
| Keywords: | Flow cytometry, polyploidy, ploidy identification, chemomutagenesis, colchicine, leaf index, guard cell size |
| Abstract:
The aim of this study was to develop a novel approach to produce autopolyploid plants of Viola x wittrockiana Gams. – Pirna group. Two variants of colchicine treatment were used to induce chromosome doubling and their efficiency was compared. Early detection of novel ploidy levels was performed by screening guard cell size, leaf index value (leaf blade length/width) and other morphological characteristics. Subsequent screening of novel ploidy levels was performed by DNA flow cytometry (FCM). | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
