ISHS Acta Horticulturae 623: XXVI International Horticultural Congress: Plant Genetic Resources, The Fabric of Horticulture's Future
CRYOPRESERVATION OF TEMPERATE FRUIT SPECIES: QUALITY OF PLANT MATERIALS AND METHODOLOGIES FOR GENE BANK CREATION |
| Authors: | C. Damiano, A. Frattarelli, M.A. Shatnawi, Y. Wu, C. Forni, F. Engelmann |
| Keywords: | biotechnology, in vitro, phenols, proteins |
| Abstract:
The Fruit Trees Institute (ISF) has implemented a research programme on the development of cryopreservation techniques for temperate fruit species. Some of these research activities have been performed in the framework of the Italian-funded IPGRI training scheme under which young researchers received specialised training in cryopreservation. Experiments have been performed with organogenic callus and suspension cultures of strawberry and apices of almond, apple, peach, kiwi and walnut. Strawberry cell suspensions have been successfully cryopreserved using a protocol including pre-treatment with a vitrification solution followed by two-step freezing. With apices of all materials tested, the encapsulation-dehydration technique consistently produced higher survival than the vitrification technique. In almond ‘Supernova’, total protein profiles of plant samples taken at different steps were done; a new band was found at 18.7 kDa after 180 days cryopreservation. This band is probably due to temporary variations of proteic pattern as a consequence of in vitro propagation. So far the plant materials have been confirmed to be true to the type after conservation. | |
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