ISHS Acta Horticulturae 623: XXVI International Horticultural Congress: Plant Genetic Resources, The Fabric of Horticulture's Future
USE OF THE CHALCONE SYNTHASE GENE INTRON IN CHARACTERIZING PETUNIA TAXA |
| Authors: | R.J. Griesbach, R.M. Beck |
| Keywords: | molecular markers, taxonomy, flavonoid |
| Abstract:
Most molecular marker studies compare DNA segments that are under selection pressure and can only measure genetic distance and not evolutionary distance. Evolutionary distance measurements requires a segment of DNA that can freely accumulate all types of mutations (point, translocation, inversion, etc.). The true relationships between species can only be determined through evolutionary distance measurements. Because the exact sequence of an intron is not needed for function, mutations can accumulate without affecting function. A method was developed to characterize the chalcone synthase gene intron. Restriction fragment length polymorphism (RFLP) and sequence analysis of several Petunia taxa is being used to develop an evolutionary tree of the gene. | |
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