ISHS Acta Horticulturae 620: XXVI International Horticultural Congress: Asian Plants with Unique Horticultural Potential: Genetic Resources, Cultural Practices, and Utilization
SHORTENING BREEDING CYCLES OF SPIDER LILIES (LYCORIS SPP.) THROUGH EMBRYO CULTURE AND DIKARYOTYPE HYBRIDIZATION BETWEEN LYCORIS AUREA AND “A” KARYOTYPE SPECIES |
| Authors: | M.S. Yuan, M.C. Wu, C.T. Shii |
| Keywords: | germination, growth, hybrid, hypogeal, in vitro culture, vigor |
| Abstract:
In spider lilies, delayed hypogeal germination and restricted growth in the current growth cycle were mainly inhibited by the surrounding tissue. The mature embryo may also limit emergence of the first true leaf, allowing one leaf emergence in the current growth cycle. Under two temperature regimes and 2.3-3.3 true leaves appeared following 3-4 months after inoculation (equal to the second year growth vigor of direct seeding). Excised embryos on MS medium without supplement of plant growth regulator were capable of inducing rapid germination in 1-3 weeks. In vitro bulblets reached maturity in 4-5 cycles compared with 5-6 cycles of direct seeding. Additionally, the interspecific hybridization between subtropical Lycoris aurea (2n=14=8M+6T) and four “A” karyotype species (2n=22A) resulted in dikaryotype hybrids (2n=18=4M+3T+11A). In combination with immature embryo rescue method, all these dikaryotype hybrids were able to produce 3-5 true leaves in current growth cycles and reached a mature stage with more than 8 true leaves by the third growth cycles. It was therefore possible to force dikaryotype bloom faster, 2-3 growth cycles shorter than parental species. Numerous subtropical genotypes with elite flowers were selected. | |
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