ISHS Acta Horticulturae 620: XXVI International Horticultural Congress: Asian Plants with Unique Horticultural Potential: Genetic Resources, Cultural Practices, and Utilization
PLANT REGENERATION FROM LEAF EXPLANTS AND EFFICIENT AGROBACTERIUM-MEDIATED TRANSFORMATION SYSTEM OF CHRYSANTHEMUM (DENDRANTHEMA GRANDIFLORUM) |
| Authors: | S.Y. Seo, D.C. Choi, J.M. Kim, H.C. Lim, H.J. Kim, J.S. Choi, Y.G. Choi |
| Keywords: | growth regulators, npt, kanamycin, PCR amplification, transgenic plant |
| Abstract:
Leaf explants of the chrysanthemum (Dendranthema grandiflorum) were cultured on MS medium supplemented with various concentrations of NAA, IAA, 2,4-D, and BA. The highest frequency of shoot formation was observed on MS medium supplemented with 1.0 mg•L-1 BA and 0.3 mg•L-1 2,4-D in ‘Puma’ and with 2.0 mg•L-1 NAA and 0.5 mg•L-1 BA in ‘Subangryeok’. The suitable concentrations of kanamycin to select the transformant are 20 mg•L-1 in ‘Puma’ and 50 mg•L-1 in ‘Subangryeok’. In the ‘Puma’, the calli formed most efficiently in the medium containing kanamycin from leaf discs co-cultivated with A. tumefaciens inoculation at 1:50 dilution ratio for 5 min. There were 700 bp npt gene bands in the two transformants through the PCR detection of npt gene. | |
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