ISHS Acta Horticulturae 619: XXVI International Horticultural Congress: Potatoes, Healthy Food for Humanity: International Developments in Breeding, Production, Protection and Utilization
CONCENTRATION AND DISTRIBUTION OF TOTAL SOLUBLE PROTEIN IN FRESH AND STORED POTATO TUBERS |
| Authors: | E. Ortiz-Medina, D.J. Donnelly |
| Keywords: | Solanum tuberosum, Bradford method, microtubers, tissue layer, periderm, cortex, pith |
| Abstract:
Total soluble protein (TSP) concentration and distribution was determined for 20 cultivars (cvs.) of both fresh and stored potato tubers from the 2000 and 2001 growing seasons. A subset of 7 cvs. was used to compare the concentration and distribution of TSP between fresh field-grown tubers and microtubers. TSP concentration was quantified separately in three tissue layers (periderm, cortex and pith) using the Bradford method. In most cvs., the TSP concentration on a dry weight (DW) basis was greater in the periderm compared with the cortex and pith. The TSP concentration in fresh field-grown tubers ranged from 38 to 73 mg g-1 DW in the periderm compared with 30 to 49 mg g-1 DW in the cortex and pith. After 6 months of tuber storage, the TSP concentration was unchanged in eleven cvs., decreased (mean of 16%) in five cvs. and increased (mean of 18%) in four cvs. While the relative TSP concentration in the tissues tended to be distributed in a similar pattern for each cv., whether fresh or stored, concentrations were greater in microtubers than in fresh field-grown tubers; possibly a function of the readily available nitrogen in the tissue culture medium. These results suggest avenues for identifying and selecting genotypes with increased protein concentrations and improved nutritive value. | |
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