ISHS Acta Horticulturae 616: I International Symposium on Acclimatization and Establishment of Micropropagated Plants
GROWTH REGULATION BY DIF REGIMES APPLIED FOR THE MICROPROPAGATION OF CHAMELAUCIUM UNCINATUM SCHAU |
| Authors: | B. Ruffoni, C. Dalla Guda, P. Restuccia, E. Farina |
| Keywords: | Architectural control, in vitro culture, temperature |
| Abstract:
Chamelaucium uncinatum was used as a model plant to evaluate the potential of the DIF technique (DIF = difference between mean day temperature and mean night temperature) at micropropagation level for the architectural control up to the final plant development in greenhouse eleven months after the treatment. Three clones were propagated. The temperature regimes during two subsequent subcultures and the rooting phase were: mean temperatures of 20 and 24°C x DIF conditions of +8, -8, 0. A 12h photoperiod (PPFD= 30 µmol sec-1m-2) was supplied by Osram lumilux white lamps. The growth media were based on the MS basal formulation. The rooted plants were acclimatized, potted and grown in greenhouse for eleven months till blooming. Multiplication rates were modified by the thermic regime (DIF level x average temperature level) but remained in a range of high values. The conditions of DIF -8 affected dry matter content negatively. The rooting percentages was unaffected by the negative DIF. At the end of the rooting phase the height of the micropropagated plant and the mean internode length reached the lowest value at DIF -8 or 0. As a general rule genotype modulated the differential response to the DIF level. Eleven months later at the onset of blooming in the greenhouse one of the three clones displayed height reduction after propagation with DIF -8 while an other clone displayed the same response after propagation with DIF 0. Blooming was regular irrespective of the DIF treatment. | |
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