ISHS Acta Horticulturae 616: I International Symposium on Acclimatization and Establishment of Micropropagated Plants
PRODUCTION OF 7-METHYLJUGLONE IN DROSERA ROTUNDIFOLIA IN VITRO AND EX VITRO |
| Authors: | K. Kämäräinen, K. Laine, A. Hohtola, J. Jalonen |
| Keywords: | Genotypes, medicinal plants, micropropagation, naphthoquinone |
| Abstract:
The round-leaved sundew Drosera rotundifolia L. produces medicinally active naphthoquinones, mainly 7-methyljuglone and plumbagin. Winter buds of seed propagated plants from different provinces in Finland were used in the initiation of culture in this experiment. After surface disinfestation treatment the plants were propagated in modified half strength MS medium, supplemented with 0.27 µM NAA and 0.44 µM BA. The plants were kept in a growth chamber at 22±2°C under a 16h photoperiod for the multiplication phase. After multiplication the plants were stored in the same medium in continuous weak light at 4°C. A portion of the plantlets were further cultivated in vitro, and rest of the material was transferred to growth pools. Fertilizers were used for part of the ex vitro material. Gas-chromatography was used for the analysis of 7-methyljuglone production. Multiplication was achieved in all the genotypes, which survived the culture initiation phase. High contamination rate was noticed. The transfer of the cloned material to the ex vitro conditions succeeded well. The analysis of 7-methyljuglone revealed some differences between different genotypes and according to fertilizer used, but no clear correlation could be seen between the same clone from in vitro and ex vitro in the production 7-methyljuglone. . | |
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