ISHS Acta Horticulturae 616: I International Symposium on Acclimatization and Establishment of Micropropagated Plants
SOMATIC EMBRYOGENESIS IN RHODODENDRON CATAWBIENSE ‘GRANDIFLORUM’ |
| Authors: | H. Vejsadová, A.S. Petrová |
| Keywords: | Cytology, histology, in vitro regeneration, somatic embryogenesis, thidiazuron |
| Abstract:
The genetic stability of regenerants in Rhododendron catawbiense Michx. ‘Grandiflorum’ and the in vitro regeneration process from leaf explants were determined. Chromosome diploid number (2n=2x=26) in actively growing shoot apices and a high degree of cultivar genetic stability were found. The effect of different concentrations of thidiazuron (TDZ) in the presence of indole-3-butyric acid (IBA), 2,4-dichlorophenoxyacetic acid (2,4-D) or 2-isopentenyladenine (2iP) on induction of embryogenic/organogenic mass was compared. Histological evidence demonstrated embryogenic capacity of mucilaginous callus at concentration 11.4-22.7 µM of thidiazuron. Somatic embryo initiation was exclusively induced with TDZ, while IBA, 2,4-D or 2iP alone did not have any effect on formation of the embryogenic structures. | |
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