ISHS Acta Horticulturae 616: I International Symposium on Acclimatization and Establishment of Micropropagated Plants
PHYSIOLOGICAL INFLUENCE OF SUCROSE ON JUVENILE AVOCADO DURING ΙN VITRO CULTIVATION AND SUBSEQUENT ΕX VITRO ACCLIMATIZATION |
| Authors: | A. Premkumar, F. Pliego, M.A. Quesada, J.A. Mercado, A. Barceló-Muñoz |
| Keywords: | Acclimatization, in vitro growth, Persea americana, photosynthetic pigments, rubisco, starch, sucrose |
| Abstract:
The present study evaluates the effect of manipulating sucrose content in the culture medium of avocado microshoots on target physiological parameters important for ex vitro acclimatization. Three different sucrose concentrations were used, 5, 30 and 50 g l-1 and the foliar contents of carbohydrates, photosynthetic pigments and nitrogenous compounds were studied in samples taken from in vitro grown and acclimatized plants. The results obtained showed that the acclimatization process exerted a greater effect in these variables than the sucrose concentration used during in vitro culture. Starch synthesis was high during acclimatization. Acclimatization reduced the total chorophyll to carotenoids ratio from 4.5 to 2.6 regardless of the initial sucrose level. Conversely, the chlorophyll a to chlorophyll b ratio was high in acclimatized leaves. A decrease in foliar N was found as result of acclimatization, but total soluble proteins were not affected neither by sucrose treatment nor by acclimatization. SDS electrophoresis analysis showed that increasing sucrose concentration in culture medium from 5 to 50 g l-1 reduced rubisco large subunit by more than 20%. | |
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