ISHS Acta Horticulturae 616: I International Symposium on Acclimatization and Establishment of Micropropagated Plants
BENEFICIAL EFFECTS OF ARTIFICIAL OXYGEN CARRIERS IN PLANT MICROPROPAGATION |
| Authors: | M.R. Davey, P. Anthony, J.B. Power, K.C. Lowe |
| Keywords: | Anti-oxidant enzymes, cryopreservation, culture medium supplements haemoglobin, perfluorochemical liquids, plant protoplasts |
| Abstract:
Cell growth and differentiation during culture demand an adequate supply of oxygen which can be provided by the use of perfluorocarbon liquids (PFCs) or haemoglobin as culture media supplements. PFCs are inert compounds capable of dissolving up to 40% by volume of oxygen. They are immiscible with aqueous solutions and can be sterilised and recycled. Haemoglobin is miscible with aqueous culture media. Oxygenated PFCs and haemoglobin have been exploited to stimulate mitotic division in cells, including those derived from isolated protoplasts, of several plant species. They also facilitate cells to overcome stress normally associated with recovery from low temperature preservation. The information relating to metabolic events associated with PFC and haemoglobin-stimulated growth is, however, limited. Nevertheless, an important attribute is that the effect of oxygenated PFCs and haemoglobin may be long-term through the promotion of shoot regeneration and multiplication. | |
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