ISHS Acta Horticulturae 616: I International Symposium on Acclimatization and Establishment of Micropropagated Plants
REGENERATION OF JOJOBA BY SOMATIC EMBRYOGENESIS: CONTROL OF EMBRYO FORMATION, POLYAMINE CONTENT AND ROLE OF THE AGE OF EXPLANTS AND GROWTH REGULATORS |
| Authors: | L. Hamama, M. Baaziz, R. Letouzé |
| Keywords: | Leaf tissue, plant regeneration, polyamines, Simmondsia chinensis |
| Abstract:
Induction, maturation and germination of somatic embryos of jojoba [Simmondsia chinensis (Link) Schneider] from leaf tissue of female plants cultured in vitro were obtained. Leaf explants were placed on their adaxial sides in Petri dishes and maintained in darkness on the half-strength Murashige and Skoog basal medium (MS/2). The use of 2,4-dichlorophenoxyacetic acid (2,4-D, 4.52 µM) with 3 cytokinins: 6-benzylaminopurine (BAP, 4.43 µM), N-(2-chloro-4pyridyl)-N'-phenylurea (CPPU, 4.03 µM) or (E)-6-[3-(trifluoromethyl)-but-2-enylamino]purine (F3iP, 3.71 µM) led to the formation of embryogenic calli and somatic embryos. After two 30-day subcultures, embryogenic calli were transferred onto MS/2 medium supplemented with different auxins and cytokinins. Somatic embryo maturation and conversion into in vitro plantlets were obtained. The histological study has shown the conformity of each stage. A correlative study was carried out to focus the effect of growth regulators, age of explants and free polyamine content during embryo induction stage. | |
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