ISHS Acta Horticulturae 616: I International Symposium on Acclimatization and Establishment of Micropropagated Plants
EFFECTS OF ROOTING CHEMICALS ON THE ESTABLISHMENT OF MICROPROPAGATED PICRORHIZA KURROA PLANTLETS IN THE GREENHOUSE |
| Authors: | C. Wawrosch, P. Zeitlhofer, B. Grauwald, B. Kopp |
| Keywords: | Acclimatization, conservation, medicinal plant, Scrophulariaceae, survival |
| Abstract:
As part of a micropropagation procedure for the endangered medicinal plant Picrorhiza kurroa (Scrophulariaceae) the influence of the rooting conditions on establishment of the plants ex vitro has been investigated. In vitro rooting was performed on MS medium supplemented with IAA, IBA or NAA at a level of 1 µM. The percentage of rooted shoots was high on all media except for the auxin free control (70%). Root length was low when IAA or NAA were used while IBA and the control induced longer roots. Basal callus was formed on all media but was not a problem when IBA was applied. Establishment of the plantlets in the greenhouse was most successful when the shoots were rooted with IBA medium (100% survival), followed by IAA (84%) and control medium (76%). Only 24% of the plants rooted with NAA survived hardening, and there was evidence that the survival and development of the plantlets correlated with the amount of basal callusing. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
