ISHS Acta Horticulturae 616: I International Symposium on Acclimatization and Establishment of Micropropagated Plants
ROOTING OF MICROCUTTINGS AND ACCLIMATIZATION IN BOUGAINVILLEA |
| Authors: | S. Hatzilazarou, M. Kotis, A.S. Economou, N. Rifaki |
| Keywords: | Bougainvillea sp. ‘Alexandra’, in vitro culture, ex vitro rooting, fog system, micropropagation, pot plant |
| Abstract:
Microcuttings of bougainvillea ‘Alexandra’ were placed for rooting in vitro in MS medium supplemented with IBA, IAA or NAA in various concentrations. The best rooting (%) was achieved with the application of IAA or NAA at 2 µΜ (41% and 36%, respectively) in 8 weeks. The inclusion of 20 or 40 g l-1 sucrose in the rooting medium did not influence the rooting percentage, while 8 and 16 µΜ IAA decreased microcutting rooting. In ex vitro rooting in the fog system or in the growth chamber, application of IAA or K-IBA decreased the percentage of rooting, while the microcuttings in the growth chamber achieved twice as much rooting percentages as compared to those in the fog system (70% and 40%, respectively). The transplanting losses during acclimatization were higher for plantlets derived from in vitro rooted microcuttings than ex vitro rooted microcuttings. Acclimatization in the fog favoured the survival rate and reduced the transplanting losses to 2%. | |
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