ISHS Acta Horticulturae 616: I International Symposium on Acclimatization and Establishment of Micropropagated Plants
ROOTING IN VITRO AND ACCLIMATIZATION OF MYRTUS COMMUNIS MICROCUTTINGS |
| Authors: | S. Hatzilazarou, H. Grammatikos, A.S. Economou, N. Rifaki, P. Ralli |
| Keywords: | Fog, hardening, microplants, micropropagation, myrtle, pot plant, shading |
| Abstract:
Microcuttings derived from in vitro cultures of two Myrtus communis clones, with large (clone A) and small (clone B) leaves were placed for rooting in WPM medium supplemented with various concentrations (0, 0.5, 1 or 2 µM) of IBA, IAA or NAA. After 8 weeks, measurements of rooting (%), number and length of the roots were taken. The best rooting was achieved with the application of 0.5 µM IBA (96% rooting) and 1 μM IAA (100% rooting) for clone A and B, respectively. The rooted microcuttings had 2-3 roots approx. 2 cm long for both clones. High concentrations of the rooting growth regulators decreased the rooting percentage. Rooted microcuttings, from both clones, were transferred to the greenhouse and transplanted to a soilless mixture in pots and placed for further growth and hardening in a fog system with full sunlight in June or shading (50%) and gradual reduction of the relative humidity from 95% to 80% in a 2-week period. The transplanting losses in microplants during acclimatization in both clones were less than 3%. | |
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