ISHS Acta Horticulturae 616: I International Symposium on Acclimatization and Establishment of Micropropagated Plants
ROOTING AND ACCLIMATIZATION OF ORNAMENTAL MYRTLE GENOTYPES |
| Authors: | B. Ruffoni, M. Airò, G. Pascella, C. Mascarello, G. Zizzo, C. Cervelli |
| Keywords: | Lighting, micropropagation, Myrtus communis, plant growth regulators |
| Abstract:
The Myrtle (‘Myrtus communis‘/ L.) is an evergreen shrub typical of the Mediterranean area; it is an interesting plant with multipurpose use. The ornamental use takes into account the production of green cut branches for indoor decoration and production of pot plants for gardening. In this species there is a great variability in the natural germplasm around the Italian Mediterranean coasts for type and size of fruit, plant architecture, leaf size and internode lenght. Axillary shoots of 9 selected genotypes have been successfully sterilised and cultured in vitro. The shoots were multiplied on MS salts and vitamins, with 0.5 mg l-1 BA and 0.2 mg l-1 IAA. There was variability among clones for multiplication rate and rooting. IAA and IBA at 0.5 mg l-1 increased the rooting percentage and evidenced a difference in the shape and length of the roots. The effect of rooting treatments in the presence of decreasing concentration of sucrose and at two different light levels was examined with the clone PA2. After 60 days best acclimatization performances were obtained with the plants treated with 15 g l-1 sucrose at 15 µmol m-2s-1. | |
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