ISHS Acta Horticulturae 616: I International Symposium on Acclimatization and Establishment of Micropropagated Plants
ROOTING AND ACCLIMATIZATION IN TRANGENIC CARNATION MICROPLANTS |
| Authors: | I.N. Valassi, A.S. Economou, A.S. Tsaftaris |
| Keywords: | Dianthus caryophyllus, hardening, microcutting, micropropagation, tissue culture, transformation |
| Abstract:
Microcuttings (microshoots) from transformed carnation (Dianthus caryophyllus L. cv. Tanga) plantlets, originated in vitro from leaf explants and carrying the bar gene with resistance to the herbicide BASTA® achieved 47% rooting in vitro in MS medium supplemented with 3 µM IBA plus 100 µg l-1 kanamycin as a selection agent. Microcuttings from non-transformed plantlets did not root at all using the same selection agent. For the acclimatization stage, the rooted microcuttings were planted to a soilless mixture in boxes and maintained in a growth chamber with controlled environmental conditions for 2 months. Young plants produced from these transformed microcuttings were moved to greenhouse and then cuttings were taken from them for rooting. Rooted cuttings, which were transplanted to pots in a soil mixture, grew successfully to plants and produced red flowers typical of the cv. Tanga. | |
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