ISHS Acta Horticulturae 616: I International Symposium on Acclimatization and Establishment of Micropropagated Plants
ROOTING AND ACCLIMATIZATION OF IN VITRO PROPAGATED OLIVE TREE MICROSHOOTS (OLEA EUROPAEA SATIVA L.) CV. CHONDROLIA CHALKIDIKIS |
| Authors: | K. Grigoriadou, M. Vasilakakis, E.P. Eleftheriou |
| Keywords: | Micropropagation, IBA, NAA, putrescine |
| Abstract:
Microshoots of the Greek olive cv. Chondrolia Chalkidikis were cultured in vitro in WPM supplemented with 4 µΜ zeatin. The effect of various auxin concentrations (IBA, NAA) on root induction was studied. Rooting percentage up to 70% was achieved by the combination of 12 µΜ ΙΒΑ + 3 µΜ ΝΑΑ although abscission of shoot tips and leaves was common. The effect of 30 °micro;Μ putrescine and 100 ppm H3PO4 addition to the medium was also tested. The percentage of rooted microshoots as well as the number of roots/microshoot were both increased by the putrescine treatment reaching up to 93% and 4%, respectively. Root length was reduced by the addition of phosphoric acid. Rooted and non-rooted shootlets were planted in peat:perilte 4:1 (by volume) under 90% RH. Survival of rooted microshoots was high (90%) while that of non-rooted ones was low (30%). Furthermore, ex vitro trials resulted in no rooted plantlets | |
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