ISHS Acta Horticulturae 616: I International Symposium on Acclimatization and Establishment of Micropropagated Plants
PERFORMANCE OF LONICERA MICROCUTTINGS AS AFFECTED BY MINERAL NUTRIENTS AND GENOTYPE |
| Author: | S.T. Karhu |
| Keywords: | Acclimatisation, ex vitro, honeysuckle, in vitro, plant propagation, tissue culture |
| Abstract:
Different honeysuckle (Lonicera) clones were propagated by axillary branching in vitro in the presence of MS or WPM macro mineral nutrients. Excised axillary shoots were rooted in vitro in the presence of indole-3-butyric acid. The microcuttings rooted well without any after-effect of propagation medium but with genotype-dependent variation. The MS mineral composition induced more callus growth on microcutting bases. The plantlets of five genotypes were subsequently transferred into peat-based substrate in plant propagator boxes for establishment and further growth and evaluated after a period of one month. Before transfer ex vitro, the plantlets originating from MS medium were bigger than those from WPM medium. This did not affect the ex vitro survival of the plantlets. One genotype showed reduced growth activity if the shoots had proliferated in the presence of WPM mineral nutrients. The size of the plantlets at the time of transfer ex vitro correlated closely with that one month later. This correlation was closer than that between the number of roots per microcutting and the ex vitro size of plants. | |
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