ISHS Acta Horticulturae 616: I International Symposium on Acclimatization and Establishment of Micropropagated Plants
ROOTING AND ACCLIMATISATION OF CHRISTMAS BUSH (CERATOPETALUM GUMMIFERUM SM.) |
| Authors: | K.A. Johnson, G. Armstrong |
| Keywords: | Australian native plant, in vitro manipulation, NSW Christmas bush |
| Abstract:
Micropropagation of Ceratopetalum gummiferum Sm. (Christmas bush) allows for rapid multiplication of selected clones for the cut flower and nursery industries. Rooting and acclimatisation of explants represents a difficult, yet critical stage in the tissue culture process. In rooting studies with full MS and indole butyric acid (IBA) and naphthalene acetic acid (NAA) at 2.5, 5, 25 and 50 µM, best rooting was achieved with 25 µM of IBA and NAA. However, there was also a massive growth of callus observed. In studies conducted with ½ MS medium with IBA and NAA at 1.25, 2.5, 5.0 µM although rooting was also achieved, the callus has also developed. The callus reduction was achieved by manipulation of MS salt levels in the media. Microcuttings of 5-6 cm in length on ¼ MS medium with 5 µM IBA, although they still developed some callus in vitro, when transplanted to the ex vitro environment survived well. Acclimatisation of explants was also problematic as the explants require high humidity, but water on leaves induced leaf and plantlet decay. Studies have been conducted in an effort to control environmental conditions, and thus enable high success rates when planting ex vitro. Best results were achieved when ‘mini-greenhouse’ was used to acclimatise Christmas bush microcuttings. | |
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