ISHS Acta Horticulturae 616: I International Symposium on Acclimatization and Establishment of Micropropagated Plants
EFFICIENCY OF A NOVEL CULTURE SYSTEM BY USING LIGHT-EMITTING DIODE (LED) ON IN VITRO AND SUBSEQUENT GROWTH OF MICROPROPAGATED BANANA PLANTLETS |
| Authors: | D.T. Nhut, T. Takamura, H. Watanabe, M. Tanaka |
| Keywords: | Acclimatization, Musa spp., in vitro growth, irradiation level |
| Abstract:
This paper covers the in vitro plantlet development of banana cultured in “Culture Pack”-rockwool system with CO2 enrichment (3000 µmol mol-1) under different light-emitting diode (LED) irradiation levels as compared to that of plant growth fluorescent lamps (PGF) and 80% red + 20% blue LED at 45 µmol m-2s-1 for 30 days. Shoot explants with three leaves were cultured on Murashige and Skoog (1962) medium with 0.02 mg l-1 IBA under different LED irradiation levels (45, 60 or 75 µmol m-2s-1) in which the red to blue ratio is 90% red + 10% blue LED as compared to that under PGF and 80% red + 20% blue LED. Attempts were also made to examine whether the light source during in vitro culture affects the subsequent growth of plantlets. Shoot and root fresh weight of in vitro plantlets grown under 60 µmol m-2s-1 was higher than that under 45, 75 µmol m-2s-1 and PGF, and the value was equal to that under 80% red + 20% blue LED while top and root fresh weight of subsequent plantlets grown under 60 µmol m-2s-1 was highest as compared to that of the others. These results suggested that novel culture system by using LED and film system was very valuable for the micropropagation of banana. | |
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