ISHS Acta Horticulturae 616: I International Symposium on Acclimatization and Establishment of Micropropagated Plants
MOLECULAR IDENTIFICATION OF MICROPROPAGATED PLANTS |
| Authors: | P.E. Read, J.E. Preece |
| Keywords: | DNA fingerprinting, genetic fidelity, molecular genetic markers, somaclonal variation, tissue culture |
| Abstract:
During the past decade the use of molecular markers in plant genetics and breeding has flourished. Molecular markers such as RAPDs, RFLPs, AFLPs, DAFs, SCARs, SSRs and ISSRs have been introduced in tissue culture research and can potentially be used in various facets of pertinent studies. This review focuses on the employment of molecular markers in micropropagated plants for the: assessment of genetic fidelity, uniformity, stability and true-to-typeness among donor plants and tissue culture regenerants; determination of the precise origin of plants derived from microspore, anther culture, or protoplast fusion; investigation of the levels and mechanisms of somaclonal variation; verification of mutant phenotypes; identification of QTLs controlling vegetative propagation and somatic embryogenesis, etc. The pertinent literature is reviewed and the relative merits and shortcomings of the various molecular markers applied are presented with an emphasis on the nature of tissue culture-induced variation, marker choice and strategies for loci to be sampled and genomes to be studied towards an adequate evaluation of related research questions. The application of genomics in the identification of micropropagated plants is also introduced. | |
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