ISHS Acta Horticulturae 610: V International Symposium on Kiwifruit
MOLECULAR MARKERS FOR EARLY SEX DETERMINATION IN ACTINIDIA |
| Authors: | X. Xiao, L. Zhang, S. Li, R. Testolin, G. Cipriani |
| Keywords: | kiwifruit, sex screening, sex identification, sex marker, AFLP, RAPD |
| Abstract:
In order to search molecular markers for early sex identification in the genus Actinidia, bulk segregant analysis and then individual analysis were performed using AFLP and RAPD markers. The segregating populations were a full sibling Actinidia deliciosa  A. eriantha hybrid family (P1) and two intra-specific cross families of diploid A. chinensis (P2 and P3). More than 33,000 well-separated bands, obtained from 520 RAPD primers and 64 AFLP primer combinations, were analysed in the male and female bulks of the 3 populations together with the parent plants.
Two RAPD primers, OPA9 and OPAI12, each produced one male-related band in P2. M450-OPA9, a band of a. 450 bp, was present in all male individuals and 1/6 female progeny.
The band M700-OPAI12 (a.700 bp) was absent in all female individuals, and present in 5/6 male progeny.
In P2, one AFLP primer pair, E-ACG/M-CAA, amplified also a male-specific band of a. 600 bp.
This band was present in all male progeny and none of the 6 female individuals.
Unfortunately, these bands were male-specific only in the P2 population did not co-segregate with the sex either in P1 and P3.
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