ISHS Acta Horticulturae 610: V International Symposium on Kiwifruit
LIPID AND POLYSACCHARIDE VARIATIONS IN ACTINIDIA DELICIOSA DURING POLLEN ONTOGENY AND GERMINATION |
| Authors: | I. Abreu, M. Oliveira |
| Keywords: | kiwifruit, pollen grain, pollen tube, sudan black B, IKI test, PAS reagent. |
| Abstract:
Sudan black B, iodine potassium iodide solution (IKI test) and periodic acid-Schiff (PAS) reagent were used for the histochemical detection of lipids, starch and polysaccharides in resin-embedded semi-thin sections and the results were confirmed at the ultra-structural level, using transmission electron microscopy. During germination, lipids and starch were hydrolysed and probably used as energy sources for the rapidly growing pollen tubes. The insoluble polysaccharides presented a similar distribution to the one observed with IKI but they were not completely consumed persisting in the cytoplasm. | |
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