ISHS Acta Horticulturae 610: V International Symposium on Kiwifruit
CHARACTERIZATION OF PSEUDOMONAS SYRINGAE PV. ACTINIDIAE ISOLATED IN KOREA AND GENETIC RELATIONSHIP AMONG CORONATINE-PRODUCING PATHOVARS BASED ON CMAU SEQUENCES |
| Authors: | H. Shim Han, E. Jung Oak, Y. Jin Koh, J.-S. Hur, J. Sung Jung |
| Keywords: | kiwifruit, canker, coronatine, phaseolotoxin, phytotoxin |
| Abstract:
Pseudomonas syringae pv. actinidiae strains which cause a canker disease of kiwifruit were isolated from vineyards in Korea. The phytotoxin produced by Korean strains was compared with that of Japanese strains, including the pathotype strain Kw11, of P. syringae pv. actinidiae. PCR primers derived from the phaseolotoxin gene cluster specifically amplified 1.4 kb fragment from DNA of Japanese strains, whereas the DNA from Korean strains did not produce the expected size of bands upon amplification. In contrast, the Korean strains amplified PCR products of 0.71 kb with the CAU primers based on cmaU gene in coronatine biosynthetic gene cluster. To determine the phytotoxin produced by Japanese and Korean strains, phaseolotoxin bioassay with Escherichia coli and coronatine bioassay with tomato leaves were separately conducted. Although all the Japanese strains tested produced phaseolotoxin, the Korean strains produced only coronatine. PCR products amplified with CAU primers and DNA from six coronatine-producing P. syringae pathovars were further characterized by nucleotide sequencing. The phylogenetic relatedness of six coronatine-producing pathovars was judged by their nucleotide sequence homologies in the amplified fragments. The phylogenetic tree indicates that the Korean strain of P. syringae pv. actinidiae and pv. glycinea seem to be closely related. | |
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