ISHS Acta Horticulturae 605: II International Symposium on Fig
IMPROVEMENT OF AN INOCULUM PRODUCTION OF PLASMOPARA VITICOLA |
| Authors: | B. Khadari, I. Hochu, S. Santoni, A. Oukabli, M. Ater, J.P. Roger, F. Kjellberg |
| Keywords: | grapevine, virus diseases, occurrence, virological screening methods, virus-free varieties |
| Abstract:
The efficiency of RAPD, ISSR and microsatellite markers for the molecular characterisation of 30 fig cultivars was determined by evaluating information content (expected heterozygosity), number of loci simultaneously analysed per experiment (multiplexing ratio), identification efficiency of a primer or locus (discriminating power). Microsatellite markers have the highest expected heterozygosity (0.64), while ISSR markers have the highest multiplexing ratio (2.85). According to discriminating power (which ranged from 0.186 to 0.961) and to optimal combination of primers necessary to identify all accessions analysed, ISSR and microsatellite markers are the most efficient tools for fig varietal identification. Advantages and limits of these molecular markers are discussed taking into account practical issues and effectiveness in analysing the genetic structure of fig germplasm. | |
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