ISHS Acta Horticulturae 605: II International Symposium on Fig
SUCROSE, CULTURE MEDIA, AND THEIR INTERACTIONS DURING IN VITRO PROLIFERATION OF 'ROXO DE VALINHOS'(FICUS CARICA L.) |
| Authors: | G.R. Brum, M. Pasqual, A.B. Silva, N.N.J. Chalfun |
| Keywords: | fig, tissues culture, micropropagation, carbon source, shoot induction |
| Abstract:
Micropropagation is just one of many tissue culture procedures in use. The applications of this technology have multiplied rapidly in recent years. This technique produces plants that are more likely to maintain genotypic and phenotypic fidelity to the original clone. It is used for essentially all the fruit plants that are micropropagated commercially at the present time. Sucrose, media culture and their interactive effects on bud proliferation rate of Ficus carica L. cv. 'Roxo de Valinhos' were studied. The sucrose concentrations (0, 15, 30 and 45 g.L-1 each were combined factorially with MS, B5, Knudson, White and WPM media culture, were tested for optimal proliferation response. Small axillary buds were removed from these branches and culture on solid medium. Those treatments rested in growth room at 27±1 °C temperature with 16 hs photoperiod in 35 µmol.m-2.s-1 light intensity. After 60 days, we made the following assumptions in our analysis: number, length and fresh matter weight of the shoots, length and fresh weight of the roots. The best results for the aerial part were obtained at WPM medium and 15g.L-1 sucrose. There wasn't root system development. | |
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