ISHS Acta Horticulturae 603: VIII International Conference on Grape Genetics and Breeding
USE OF MOLECULAR MARKERS TO CHECK THE VARIETAL HOMOGENEITY OF BATCHES OF CUTTINGS |
| Authors: | N. Lanier, O. Larose, A. Fonda, P. Parra, P. This, S. Grenan |
| Keywords: | microsatellites, DNA, varietal identification, wood and leaf mixes, contaminating variety |
| Abstract:
The objective of this study is to try to answer to preoccupations of the nurseries. The aim is to place at the disposal of the propagation industry, a practical method to verify the varietal homogeneity of batches of cuttings or plants before their commercialization. To this aim, we tested the method of analysis by microsatellites markers that allows DNA testing for mixtures of two varieties. (Siret et al. 2002). We have first optimized the detection conditions of the contaminating variety. The detection of known wood DNA mixtures is possible until dilution to 1/30. The same applies to the detection of known leaf mixtures. However, results are however depend on the type of mixture and even more on the choice of primer. We show that automation of the analysis of leaf mixture is possible. The set up of a reliable technique for wood mixture is in progress. First blind tests of mixture analyses are not satisfactory. Conditions for a reliable commercial method need to be searched. | |
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