ISHS Acta Horticulturae 603: VIII International Conference on Grape Genetics and Breeding
ISOENZYMATIC CHARACTERISATION OF SOME GRAPEVINE CULTIVARS BRED IN BADACSONY |
| Authors: | G. Györffyné Jahnke, J. Korbuly, J. Májer |
| Keywords: | isoenzyme, isozyme, cultivar identification, molecular markers, PAGE |
| Abstract:
We used isoenzymatic analysis for the characterisation of grapevine cultivars bred in Badacsony. Vertical poliacrylamide-gel electrophoresis was employed to investigate 20 Vitis vinifera cultivars. Nine of them are in Badacsony bred hybrids (4 before state registration, 5 state registered), eight of them are the parents of one of the 9 hybrids, and the other 3 are known, in Hungary quite important cultivars for the basis of comparison. We used 3 gel-buffer systems to separate the soluble isoenzymes of catechol-oxidase (CO), peroxidase (PER), acid phosphatase (AcP), and esterase (EST). We can established, that the isoenzymatic characterisation of grapevine cultivars, with the combination of different enzyme systems, can be suitable for cultivar identification as well. | |
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