ISHS Acta Horticulturae 603: VIII International Conference on Grape Genetics and Breeding
STRUCTURE AND TRANSCRIPTION OF THREE CHALCONE SYNTHASE GENES OF GRAPEVINE (VITIS VINIFERA |
| Authors: | N. Goto-Yamamoto, G.H. Wan, S. Kobayashi |
| Keywords: | CHS; Anthocyanin; StSy; Vitis vinifera |
| Abstract:
Chalcone synthase catalyzes the first step for the biosynthesis of anthocyanin. To reveal the details of the gene family of the chalcone synthase of grapevine, three genomic clones of the chalcone synthase gene, Chs1 (AB015872), Chs2 (AB066275), and Chs3 (AB066274), were obtained from Vitis vinifera cv. Cabernet Sauvignon. The homology between Chs1 and Chs2 (96% on the amino acid level) was higher than the homologies between Chs3 and Chs1 and Chs3 and Chs2 (89% each). Phylogenetic analysis showed that Chs3 was more closely related to morning glory Chs-E and petunia Chs-A than to Chs1 and Chs2. Thus, among the three Chss of grapevine, Chs1 and Chs2 formed a subfamily, and Chs3 was divergent from them. RT-PCR analysis showed that the mRNA of Chs3 accumulated mainly in the berry skin of red cultivars during coloration, while the mRNA of Chs1 and Chs2 accumulated in the leaves and berry skin of a white cultivar as well as red cultivars. Thus, the three Chss of grapevine seemed to be under a different transcription control. | |
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