ISHS Acta Horticulturae 603: VIII International Conference on Grape Genetics and Breeding
DEVELOPMENT OF GRAPEVINE RETROTRANSPOSON-BASED MOLECULAR MARKERS (S-SAP) |
| Authors: | F. Pelsey, L. Schehrer, D. Merdinoglu |
| Keywords: | Vitis vinifera, genetic diversity, DNA fingerprinting, Ty1 copia-like retrotransposon. |
| Abstract:
Derived from the multiplex marker system AFLP, we have developed for the grapevine genome, sequence-specific amplification polymorphism (S-SAP) markers, based on the location of retrotransposons relative to adjacent restriction endonuclease sites. Insertional polymorphisms of three grapevine retrotransposons have been assessed within a set of 16 accessions of grapevine (species and varieties). Efficiency of the S-SAP fingerprinting has been compared to microsatellite markers. S-SAPs enable to distinguish between all of the tested genotypes. The topology of each resulting phenogram is unique, however, with obvious similarities. | |
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