ISHS Acta Horticulturae 603: VIII International Conference on Grape Genetics and Breeding
OPTIMIZATION OF CONDITIONS FOR REGENERATION AND GENETIC TRANSFORMATION OF ROOTSTOCK- AND SCION GRAPE VARIETIES |
| Authors: | R. Oláh, E. Szegedi, S. Ruthner, J. Korbuly |
| Keywords: | thidiazuron (TDZ), benzyladenine (BA), somatic embryogenesis, Vitis, Agrobacterium tumefaciens, nptII gene, gusA gene |
| Abstract:
Embryogenic calli have been produced from 19 of the 41 grapevine cultivars tested, including internationally grown and traditional Hungarian varieties, novel candidates, as well as rootstock cultivars. We have applied a simple yet effective transformation method with optimized conditions, which was tested in Agrobacterium-mediated transformation using an nptII/gusA gene construct. The putative transgenic lines expressed the gusA gene. T-DNA transfer and integration was further confirmed by PCR analysis of these plants. Further research efforts are in progress to introduce economically useful genes as well into grapevines. | |
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