ISHS Acta Horticulturae 603: VIII International Conference on Grape Genetics and Breeding
GENETIC ANALYSIS OF THE GRAPEVINE CULTIVAR 'PICOLIT' BASED ON MICROSATELLITE AND AFLP MARKERS |
| Authors: | L. Zulini, E. Fabro, E. Peterlunger |
| Keywords: | Vitis vinifera, SSR, Simple Sequence Repeat, fingerprinting, chimerism, clonal selection |
| Abstract:
‘Picolit’ is an ancient, female-flower cultivar with white berry, autochthonous in the region Friuli Venezia Giulia, North-Eastern Italy. The cultivar is processed to obtain high-quality dessert wines, sometimes from overripe and wilted grapes. The phenotypic and genotypic variability of this cultivar was investigated by means of ampelographic and ampelometric descriptors and the use of molecular markers, such as microsatellites and AFLPs. In the area where the cultivar is grown, i.e. the AOC “Colli Orientali del Friuli” (Friuli Eastern hills), 39 samples were collected from old plants (30 to 100 years old), which showed some morphological and growth-habit differences. The ampelographic and ampelometric analyses pointed out morphological differences in two samples (P6 and P7). These samples showed a different allelic profile at 18 out of the 21 SSR analysed and were therefore considered not belonging to the cultivar Picolit. Of the remaining samples, 35 gave the same allelic pattern at all SSRs and were therefore considered ‘true-to-type’ Picolit, whereas two of them (P4 and P8) showed several variations, including extra alleles. The possible causes of such differences are discussed, focusing in particular on the phenomenon of chimerism. The AFLP analysis, from which samples P6 and P7 were excluded, enabled screening a larger portion of the genome and confirmed the differences of P4 and P8 samples from the remaining ones. P4 and P8 were different from the majority of samples at 13 and 37 AFLP loci respectively. A few further polymorphic bands were recorded in the remaining samples, but, since they were not always reproducible, they have been disregarded. This research confirmed the appreciable somatic stability of SSR markers even in long-lived, vegetatively propagated plants, and the occasional occurrence of solid mutations and chimerisms. | |
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